Direct assessment of the metabolic origin of carbon atoms in glutamate from illuminated leaves using (13) C-NMR.
Identifieur interne : 000E19 ( Main/Exploration ); précédent : 000E18; suivant : 000E20Direct assessment of the metabolic origin of carbon atoms in glutamate from illuminated leaves using (13) C-NMR.
Auteurs : Cyril Abadie [Australie] ; Jérémy Lothier [France] ; Edouard Boex-Fontvieille [France] ; Adam Carroll [Australie] ; Guillaume Tcherkez [Australie]Source :
- The New phytologist [ 1469-8137 ] ; 2017.
Abstract
Glutamate (Glu) is the cornerstone of nitrogen assimilation and photorespiration in illuminated leaves. Despite this crucial role, our knowledge of the flux to Glu de novo synthesis is rather limited. Here, we used isotopic labelling with (13) CO2 and (13) C-NMR analyses to examine the labelling pattern and the appearance of multi-labelled species of Glu molecules to trace the origin of C-atoms found in Glu. We also compared this with (13) C-labelling patterns in Ala and Asp, which reflect citrate (and thus Glu) precursors, that is, pyruvate and oxaloacetate. Glu appeared to be less (13) C-labelled than Asp and Ala, showing that the Glu pool was mostly formed by 'old' carbon atoms. There were modest differences in intramolecular (13) C-(13) C couplings between Glu C-2 and Asp C-3, showing that oxaloacetate metabolism to Glu biosynthesis did not involve C-atom redistribution by the Krebs cycle. The apparent carbon allocation increased with carbon net photosynthesis. However, when expressed relative to CO2 fixation, it was clearly higher at low CO2 while it did not change in 2% O2 , as compared to standard conditions. We conclude that Glu production from current photosynthetic carbon represents a small flux that is controlled by the gaseous environment, typically upregulated at low CO2 .
DOI: 10.1111/nph.14719
PubMed: 28771732
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000092
- to stream PubMed, to step Curation: 000092
- to stream PubMed, to step Checkpoint: 000092
- to stream Ncbi, to step Merge: 004C33
- to stream Ncbi, to step Curation: 004C33
- to stream Ncbi, to step Checkpoint: 004C33
- to stream Main, to step Merge: 000E14
- to stream Main, to step Curation: 000E19
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Direct assessment of the metabolic origin of carbon atoms in glutamate from illuminated leaves using (13) C-NMR.</title><author><name sortKey="Abadie, Cyril" sort="Abadie, Cyril" uniqKey="Abadie C" first="Cyril" last="Abadie">Cyril Abadie</name><affiliation wicri:level="1"><nlm:affiliation>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601</wicri:regionArea><wicri:noRegion>2601</wicri:noRegion></affiliation></author><author><name sortKey="Lothier, Jeremy" sort="Lothier, Jeremy" uniqKey="Lothier J" first="Jérémy" last="Lothier">Jérémy Lothier</name><affiliation wicri:level="4"><nlm:affiliation>IRHS, Agrocampus-Ouest, INRA, Université d'Angers, SFR 4207 QuaSaV, 42 Rue Georges Morel, Beaucouzé, 49071, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IRHS, Agrocampus-Ouest, INRA, Université d'Angers, SFR 4207 QuaSaV, 42 Rue Georges Morel, Beaucouzé, 49071</wicri:regionArea><wicri:noRegion>49071</wicri:noRegion><orgName type="university">Université d'Angers</orgName><placeName><settlement type="city">Angers</settlement><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author><author><name sortKey="Boex Fontvieille, Edouard" sort="Boex Fontvieille, Edouard" uniqKey="Boex Fontvieille E" first="Edouard" last="Boex-Fontvieille">Edouard Boex-Fontvieille</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Police Scientifique de Lyon, Institut National de Police Scientifique, 31 Avenue Franklin Roosevelt, Écully Cedex, 69134, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Police Scientifique de Lyon, Institut National de Police Scientifique, 31 Avenue Franklin Roosevelt, Écully Cedex, 69134</wicri:regionArea><wicri:noRegion>69134</wicri:noRegion><wicri:noRegion>69134</wicri:noRegion></affiliation></author><author><name sortKey="Carroll, Adam" sort="Carroll, Adam" uniqKey="Carroll A" first="Adam" last="Carroll">Adam Carroll</name><affiliation wicri:level="1"><nlm:affiliation>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601</wicri:regionArea><wicri:noRegion>2601</wicri:noRegion></affiliation></author><author><name sortKey="Tcherkez, Guillaume" sort="Tcherkez, Guillaume" uniqKey="Tcherkez G" first="Guillaume" last="Tcherkez">Guillaume Tcherkez</name><affiliation wicri:level="1"><nlm:affiliation>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601</wicri:regionArea><wicri:noRegion>2601</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28771732</idno><idno type="pmid">28771732</idno><idno type="doi">10.1111/nph.14719</idno><idno type="wicri:Area/PubMed/Corpus">000092</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000092</idno><idno type="wicri:Area/PubMed/Curation">000092</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000092</idno><idno type="wicri:Area/PubMed/Checkpoint">000092</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000092</idno><idno type="wicri:Area/Ncbi/Merge">004C33</idno><idno type="wicri:Area/Ncbi/Curation">004C33</idno><idno type="wicri:Area/Ncbi/Checkpoint">004C33</idno><idno type="wicri:Area/Main/Merge">000E14</idno><idno type="wicri:Area/Main/Curation">000E19</idno><idno type="wicri:Area/Main/Exploration">000E19</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Direct assessment of the metabolic origin of carbon atoms in glutamate from illuminated leaves using (13) C-NMR.</title><author><name sortKey="Abadie, Cyril" sort="Abadie, Cyril" uniqKey="Abadie C" first="Cyril" last="Abadie">Cyril Abadie</name><affiliation wicri:level="1"><nlm:affiliation>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601</wicri:regionArea><wicri:noRegion>2601</wicri:noRegion></affiliation></author><author><name sortKey="Lothier, Jeremy" sort="Lothier, Jeremy" uniqKey="Lothier J" first="Jérémy" last="Lothier">Jérémy Lothier</name><affiliation wicri:level="4"><nlm:affiliation>IRHS, Agrocampus-Ouest, INRA, Université d'Angers, SFR 4207 QuaSaV, 42 Rue Georges Morel, Beaucouzé, 49071, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>IRHS, Agrocampus-Ouest, INRA, Université d'Angers, SFR 4207 QuaSaV, 42 Rue Georges Morel, Beaucouzé, 49071</wicri:regionArea><wicri:noRegion>49071</wicri:noRegion><orgName type="university">Université d'Angers</orgName><placeName><settlement type="city">Angers</settlement><region type="region" nuts="2">Pays de la Loire</region></placeName></affiliation></author><author><name sortKey="Boex Fontvieille, Edouard" sort="Boex Fontvieille, Edouard" uniqKey="Boex Fontvieille E" first="Edouard" last="Boex-Fontvieille">Edouard Boex-Fontvieille</name><affiliation wicri:level="1"><nlm:affiliation>Laboratoire de Police Scientifique de Lyon, Institut National de Police Scientifique, 31 Avenue Franklin Roosevelt, Écully Cedex, 69134, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Police Scientifique de Lyon, Institut National de Police Scientifique, 31 Avenue Franklin Roosevelt, Écully Cedex, 69134</wicri:regionArea><wicri:noRegion>69134</wicri:noRegion><wicri:noRegion>69134</wicri:noRegion></affiliation></author><author><name sortKey="Carroll, Adam" sort="Carroll, Adam" uniqKey="Carroll A" first="Adam" last="Carroll">Adam Carroll</name><affiliation wicri:level="1"><nlm:affiliation>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601</wicri:regionArea><wicri:noRegion>2601</wicri:noRegion></affiliation></author><author><name sortKey="Tcherkez, Guillaume" sort="Tcherkez, Guillaume" uniqKey="Tcherkez G" first="Guillaume" last="Tcherkez">Guillaume Tcherkez</name><affiliation wicri:level="1"><nlm:affiliation>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Research School of Biology, College of Medicine, Biology and Environment, Australian National University, Canberra, ACT, 2601</wicri:regionArea><wicri:noRegion>2601</wicri:noRegion></affiliation></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Glutamate (Glu) is the cornerstone of nitrogen assimilation and photorespiration in illuminated leaves. Despite this crucial role, our knowledge of the flux to Glu de novo synthesis is rather limited. Here, we used isotopic labelling with (13) CO2 and (13) C-NMR analyses to examine the labelling pattern and the appearance of multi-labelled species of Glu molecules to trace the origin of C-atoms found in Glu. We also compared this with (13) C-labelling patterns in Ala and Asp, which reflect citrate (and thus Glu) precursors, that is, pyruvate and oxaloacetate. Glu appeared to be less (13) C-labelled than Asp and Ala, showing that the Glu pool was mostly formed by 'old' carbon atoms. There were modest differences in intramolecular (13) C-(13) C couplings between Glu C-2 and Asp C-3, showing that oxaloacetate metabolism to Glu biosynthesis did not involve C-atom redistribution by the Krebs cycle. The apparent carbon allocation increased with carbon net photosynthesis. However, when expressed relative to CO2 fixation, it was clearly higher at low CO2 while it did not change in 2% O2 , as compared to standard conditions. We conclude that Glu production from current photosynthetic carbon represents a small flux that is controlled by the gaseous environment, typically upregulated at low CO2 .</div></front></TEI><affiliations><list><country><li>Australie</li><li>France</li></country><region><li>Pays de la Loire</li></region><settlement><li>Angers</li></settlement><orgName><li>Université d'Angers</li></orgName></list><tree><country name="Australie"><noRegion><name sortKey="Abadie, Cyril" sort="Abadie, Cyril" uniqKey="Abadie C" first="Cyril" last="Abadie">Cyril Abadie</name></noRegion><name sortKey="Carroll, Adam" sort="Carroll, Adam" uniqKey="Carroll A" first="Adam" last="Carroll">Adam Carroll</name><name sortKey="Tcherkez, Guillaume" sort="Tcherkez, Guillaume" uniqKey="Tcherkez G" first="Guillaume" last="Tcherkez">Guillaume Tcherkez</name></country><country name="France"><region name="Pays de la Loire"><name sortKey="Lothier, Jeremy" sort="Lothier, Jeremy" uniqKey="Lothier J" first="Jérémy" last="Lothier">Jérémy Lothier</name></region><name sortKey="Boex Fontvieille, Edouard" sort="Boex Fontvieille, Edouard" uniqKey="Boex Fontvieille E" first="Edouard" last="Boex-Fontvieille">Edouard Boex-Fontvieille</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E19 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000E19 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Asie
|area= AustralieFrV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:28771732
|texte= Direct assessment of the metabolic origin of carbon atoms in glutamate from illuminated leaves using (13) C-NMR.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:28771732" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AustralieFrV1
| This area was generated with Dilib version V0.6.33. |  |